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KMID : 1059519970410090471
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Journal of the Korean Chemical Society
1997 Volume.41 No. 9 p.471 ~ p.482
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Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry
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Yoo Eun-Ah
Ko Sun-Young
Kim Jong-Won
Kim Jeong-Uk
Myung Seung-Woon
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Abstract
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GC/MS was used to find an optimum experimental condition for the screening of organic acids in urine. Urinary organic acids were isolated through the liquid-liquid extraction method (LLE) to examine the influence of pH and the effect of including the back extraction and oximation processes respectively on the extraction. When pH was adjusted to 0.5 during the extraction without oximation process, relatively higher recovery rate and the smallest relative standard deviations (0.3-12.4%) were obtained. By removing the interference, the addition of back extraction made possible surer identification of organic acids with retention time of 15-16 minutes. Under this condition, we obtained the content distribution of urinary organic acids in healthy Korean children (n=16) by establishing the calibration curves for 51 standard organic acids.
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KEYWORD
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